1ZXM
Human Topo IIa ATPase/AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2004-05-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.772, 75.285, 164.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.870 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.078 | |
Number of reflections | 70576 | |
<I/σ(I)> | 15.7 | |
Completeness [%] | 98.5 | 96.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 277 | PEG 6000, Tris chloride, magnesium chloride, DTT, BME, AMP-PNP., pH 7.4, VAPOR DIFFUSION, temperature 277K |