1ZSV
Crystal structure of human NADP-dependent leukotriene B4 12-hydroxydehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98402 |
| Spacegroup name | P 43 |
| Unit cell lengths | 92.543, 92.543, 202.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.300 - 2.300 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.24933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1V3V.pdb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.618 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.139 | |
| Number of reflections | 74944 | |
| Completeness [%] | 99.4 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG3350, NaI, ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
