1ZM7
Crystal structure of D. melanogaster deoxyribonucleoside kinase mutant N64D in complex with dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.040, 119.270, 68.388 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 44.720 - 2.200 |
| R-factor | 0.21329 |
| Rwork | 0.212 |
| R-free | 0.23695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.274 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | |
| Number of reflections | 65654 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | MES, lithium sulphate, mPEG 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
