1ZLT
Crystal Structure of Chk1 Complexed with a Hymenaldisine Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-01-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.000, 65.643, 58.112 |
| Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.740 |
| Rwork | 0.227 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nvq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 34070 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 76.5 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | PEG8000, ammonium sulfate, cacodylate, glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP |
