1Z3N
Human aldose reductase in complex with NADP+ and the inhibitor lidorestat at 1.04 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.140, 67.340, 47.880 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.040 |
| R-factor | 0.098 |
| Rwork | 0.091 |
| R-free | 0.12500 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.060 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Number of reflections | 139636 | |
| <I/σ(I)> | 12.4 | 4.05 |
| Completeness [%] | 87.2 | 85.4 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | Aldose Reductase, Lidorestat, NADP+, PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
