1Z2B
Tubulin-colchicine-vinblastine: stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC |
| Spacegroup name | P 65 |
| Unit cell lengths | 324.852, 324.852, 54.208 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.100 |
| R-factor | 0.21243 |
| Rwork | 0.209 |
| R-free | 0.26886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.906 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.040 | 0.330 |
| Number of reflections | 25286 | |
| <I/σ(I)> | 25 | 2.5 |
| Completeness [%] | 94.8 | 76.6 |
| Redundancy | 3.26 | 2.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | PEG, PIPES BUFFER, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K. THE CRYSTAL OF TUBULIN-COLCHICINE:RB3-SLD COMPLEX WAS SOAKED WITH A 2 MILLI-MOLAR VINBLASTINE SOLUTION FOR 24 HOURS., pH 7.00 |
