1YZZ
Humanized caban33 at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2005-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.473, 52.473, 165.365 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.238 |
| Rwork | 0.233 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yc8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.108 | 0.558 |
| Number of reflections | 9871 | |
| <I/σ(I)> | 24.6 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.46 | 14.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG4000, ammonium sulphate, TRIS, NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
