1YW2

Mutated Mus Musculus P38 Kinase (mP38)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-19
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	65.036, 76.954, 73.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.010
R-factor	0.207
Rwork	0.207
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cm8
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.010	2.070
Rmerge	0.062
Number of reflections	24876
<I/σ(I)>	19
Completeness [%]	97.7	95.2
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287	PEG 1500, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K