1YVJ
Crystal structure of the Jak3 kinase domain in complex with a staurosporine analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.307, 54.215, 118.607 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.970 - 2.550 |
R-factor | 0.2041 |
Rwork | 0.204 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m17 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.970 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 9949 | |
Completeness [%] | 97.9 | 97.4 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | malonate, glycerol, pipes, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |