1YTU
Structural basis for 5'-end-specific recognition of the guide RNA strand by the A. fulgidus PIWI protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9776 |
| Spacegroup name | H 3 |
| Unit cell lengths | 238.747, 238.747, 52.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.20689 |
| Rwork | 0.204 |
| R-free | 0.26623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w9h |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.046 | 0.279 |
| Number of reflections | 38254 | |
| Completeness [%] | 99.3 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4K, potassium chloride, magnisium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4K | ||
| 2 | 1 | 1 | potassium chloride | ||
| 3 | 1 | 1 | magnisium chloride | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | potassium chloride | ||
| 7 | 1 | 2 | magnisium chloride | ||
| 8 | 1 | 2 | PEG 4K |
