1YGU
Crystal structure of the tandem phosphatase domains of RPTP CD45 with a pTyr peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-03 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.900, 57.920, 159.290 |
Unit cell angles | 90.00, 98.66, 90.00 |
Refinement procedure
Resolution | 29.160 - 2.900 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D2 domain of RPTP LAR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.273 | |
Number of reflections | 32025 | |
<I/σ(I)> | 17.9 | 2.1 |
Completeness [%] | 92.8 | 67 |
Redundancy | 3.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 4000, sodium acetate, glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |