1YFJ
T4Dam in Complex with AdoHcy and 15-mer Oligonucleotide Showing Semi-specific and Specific Contact
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.200, 133.000, 189.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.780 - 2.690 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q0s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 78518 | |
| <I/σ(I)> | 12.9 | 7.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.3 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | PEG 6000, MES, ammonium acetate, CaCl2, and ethyleneglycol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | ammonium acetate | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | ethyleneglycol | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 6000 | ||
| 8 | 1 | 2 | ammonium acetate | ||
| 9 | 1 | 2 | CaCl2 |
