1YC3
Crystal Structure of human HSP90alpha complexed with dihydroxyphenylpyrazoles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-19 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.775, 44.829, 53.157 |
Unit cell angles | 90.00, 114.62, 90.00 |
Refinement procedure
Resolution | 48.330 - 2.120 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.330 | 2.250 |
High resolution limit [Å] | 2.100 | 2.120 |
Rmerge | 0.114 | 0.320 |
Number of reflections | 12896 | |
<I/σ(I)> | 10.2 | |
Completeness [%] | 99.2 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 277 | PEG 6000, LiCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 9.00 |