1YA3
Crystal structure of the human mineralocorticoid receptor ligand-binding domain bound to progesterone and harboring the S810L mutation responsible for a severe form of hypertension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2004-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 31 |
| Unit cell lengths | 120.724, 120.724, 42.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.720 - 2.340 |
| R-factor | 0.252 |
| Rwork | 0.237 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y9r |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.730 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.076 | 0.311 |
| Number of reflections | 22222 | |
| <I/σ(I)> | 8.6 | 2.8 |
| Completeness [%] | 81.7 | 80.3 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
