1Y97
The human TREX2 3' exonuclease structure suggests a mechanism for efficient non-processive DNA catalysis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-30 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.97832, 0.97876, 0.9500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.500, 77.200, 101.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.26100 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.550 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.078 | 0.180 |
Number of reflections | 14344 | |
<I/σ(I)> | 19 | 4 |
Completeness [%] | 97.0 | 80 |
Redundancy | 5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 303 | 0.4 M ammonium phosphate, 7% PEG400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 303K |