1Y5R
The crystal structure of murine 11b-hydroxysteroid dehydrogenase complexed with corticosterone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 96.051, 96.051, 218.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
Rwork | 0.210 |
R-free | 0.24700 |
Structure solution method | SIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.763 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.068 | 0.250 |
Number of reflections | 21330 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 1.8M Li2SO4, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, temperature 293K |