1XWF
K185N mutated S-adenosylhomocysteine hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.340, 109.370, 104.070 |
| Unit cell angles | 90.00, 115.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 44886 | |
| Completeness [%] | 100.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG4000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
