1XSN
Crystal Structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.96318 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.043, 62.551, 139.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.840 - 1.950 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 34547 | |
<I/σ(I)> | 15.7 | |
Completeness [%] | 94.9 | 95.2 |
Redundancy | 7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Sodium Citrate, 2-propanol, sodium chloride, cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Citrate | ||
2 | 1 | 1 | 2-propanol | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | cacodylate |