1XLX

Catalytic Domain Of Human Phosphodiesterase 4B In Complex With Cilomilast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	93
Detector technology	CCD
Collection date	2003-08-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	89.274, 94.259, 106.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.710 - 2.190
R-factor	0.23493
Rwork	0.232
R-free	0.28225
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.994
Data scaling software	ELVES
Phasing software	EPMR
Refinement software	REFMAC (5.1.25)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.710	2.247
High resolution limit [Å]	2.190	2.190
Rmerge	0.072	0.666
Number of reflections	41167
<I/σ(I)>	6.6	1.1
Completeness [%]	92.8	95.9
Redundancy	4.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	277	ammonium sulfate and lithium sulfate , pH 10, VAPOR DIFFUSION, SITTING DROP, temperature 277K