1XHY
X-ray structure of the Y702F mutant of the GluR2 ligand-binding core (S1S2J) in complex with kainate at 1.85 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8110 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.727, 60.300, 48.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.370 - 1.850 |
| Rwork | 0.154 |
| R-free | 0.20656 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fw0 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.747 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.400 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.432 |
| Number of reflections | 24433 | |
| <I/σ(I)> | 16.1 | 3 |
| Completeness [%] | 98.9 | 96 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 280 | PEG1000, Li2SO4, cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
