1WZY
Crystal structure of human ERK2 complexed with a pyrazolopyridazine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-20 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.599, 68.541, 59.833 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 11.960 - 2.500 |
| R-factor | 0.253 |
| Rwork | 0.249 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.700 |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNX |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.590 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 12848 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 5000, ammonium sulphate, dithiothreitol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
