1WXZ

Crystal structure of adenosine deaminase ligated with a potent inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-01
Detector	RIGAKU RAXIS
Wavelength(s)	0.8266
Spacegroup name	P 43 21 2
Unit cell lengths	77.800, 77.800, 137.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.430 - 2.800
R-factor	0.22
Rwork	0.215
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	AMoRE
Refinement software	CNX (2002)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.400
High resolution limit [Å]	2.800
Number of reflections	10708
Completeness [%]	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Ammonium sulphate, PEG400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K