1WPL
Crystal structure of the inhibitory form of rat GTP cyclohydrolase I/GFRP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.2820 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 121.720, 109.670, 130.270 |
Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
Rwork | 0.207 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1is7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.417 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.107 | 0.465 |
Number of reflections | 83700 | |
<I/σ(I)> | 7.6 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | 2-propanol, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |