1W7K
E.coli FolC in complex with ADP, without folate substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-19 |
Detector | MACSCIENCE DIP2000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.112, 81.602, 93.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.160 - 2.100 |
Rwork | 0.177 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w78 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.200 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.110 | 0.290 |
Number of reflections | 29894 | |
<I/σ(I)> | 5.8 | 2.5 |
Completeness [%] | 99.1 | 100 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.7 | 1.5 M AMMONIUM SULFATE 5 MM DTT 50 MM BICINE PH 8.7 |