1W78

E.coli FolC in complex with DHPP and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-11-06
Detector	MACSCIENCE DIP2000
Spacegroup name	P 21 21 21
Unit cell lengths	64.368, 80.620, 92.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.820
Rwork	0.165
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbv
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.920
High resolution limit [Å]	1.820	1.820
Rmerge	0.070	0.280
Number of reflections	42097
<I/σ(I)>	12.3	3.7
Completeness [%]	96.4	88.7
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.7		1.5 M AMMONIUM SULFATE 5 MM DTT 50 MM BICINE PH 8.7