1W63
AP1 clathrin adaptor core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 178.140, 178.140, 1134.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 4.000 |
| R-factor | 0.2972 |
| Rwork | 0.297 |
| R-free | 0.30970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1GW5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.861 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.840 | 4.100 |
| High resolution limit [Å] | 4.010 | 4.000 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 141015 | |
| <I/σ(I)> | 9.4 | 2.5 |
| Completeness [%] | 81.3 | 69.7 |
| Redundancy | 6.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 8-9% ETHANOL, 0.1 M NA HEPES, PH 7.5, 2 MM CYSTEINE |
