1VKJ
Crystal structure of heparan sulfate 3-O-sulfotransferase isoform 1 in the presence of PAP
Replaces: 1S6TExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97948 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 300.136, 300.136, 84.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.840 - 2.500 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nst |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 60048 | |
| <I/σ(I)> | 7.4 | 1.7 |
| Completeness [%] | 83.6 | |
| Redundancy | 11.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | .1M citrate, PEG 4K, .1M NaCl, 4mM PAP, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
