1VFN
PURINE NUCLEOSIDE PHOSPHORYLASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 94.110, 94.110, 94.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ulb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.100 |
| Rmerge | 0.074 | 0.212 |
| Number of reflections | 15158 | |
| <I/σ(I)> | 10.6 | 6 |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 18 * | 16%(W/V) PEG 4000, 0.04M TRIS-HCL BUFFER PH 8.2-8.5, 0.08M MGCL2, pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PNP-hypoxanthine | 6.6-8 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 6.6 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 0.6 (mM) | |
| 4 | 1 | drop | PEG4000 | 3.6-5.3 (%(w/v)) | |
| 5 | 1 | drop | Tris-HCl | 0.01 (M) | |
| 6 | 1 | drop | 0.03 (M) | ||
| 7 | 1 | reservoir | PEG4000 | 11-16 (%(w/v)) | |
| 8 | 1 | reservoir | Tris-HCl | 0.04 (M) | |
| 9 | 1 | reservoir | 0.08 (M) |
