1V8K

The Crystal Structure of the Minimal Functional Domain of the Microtubule Destabilizer KIF2C Complexed with Mg-AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2003-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9800
Spacegroup name	C 2 2 2
Unit cell lengths	61.068, 191.475, 74.627
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 * - 2.250
R-factor	0.215
Rwork	0.215
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	24.300 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.390
High resolution limit [Å]	2.250 *	2.250
Rmerge	0.086 *
Number of reflections	20584
Completeness [%]	98.5 *	86.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	24 *	potassium sodium tartrate tetrahydrate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	potassium sodium tartrate tetrahydrate	1.0 (M)
3	1	reservoir	MES	0.1 (M)	pH6.0