1V0C
Structure of AAC(6')-Ib in complex with Kanamycin C and AcetylCoA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-09 |
| Detector | RIGAKU-MSC RAXIS-IV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.399, 57.399, 147.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.440 - 2.200 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 13101 | |
| <I/σ(I)> | 49.4 | 18 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 15.7 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.75 | PROTEIN (10 MG/ML, 20 MM TRIS PH 7.5, 20 MM NACL, 3 MM ACCOA) PRECIPITANT (20% PEG3350, 200 MM CALCIUM ACETATE, 100 MM NACACODYLATE PH 6.75) VAPOR DIFFUSION UNDER OIL |
