1UXP
Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-08-15 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 185.750, 185.750, 135.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.550 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ky8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.650 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.046 | 0.380 |
Number of reflections | 43262 | |
<I/σ(I)> | 31 | 4 |
Completeness [%] | 95.0 | 95 |
Redundancy | 7.7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 2M SODIUMFORMATE PH 5.6, 0.1 M SODIUM ACETATE PH 5.6, 50 MM ADP |