1URQ
Crystal structure of neuronal Q-SNAREs in complex with R-SNARE motif of Tomosyn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.054, 35.602, 93.541 |
| Unit cell angles | 90.00, 132.38, 90.00 |
Refinement procedure
| Resolution | 19.389 - 2.000 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n7s |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.390 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.145 |
| Number of reflections | 21027 | |
| <I/σ(I)> | 19 | 6.4 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 30% MPD, 50MM CACL2, 50MM MES PH 6.0 |
