1UPT
Structure of a complex of the golgin-245 GRIP domain with Arl1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97925,0.97942,0.93928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.497, 89.711, 72.534 |
| Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
| Resolution | 67.000 * - 1.700 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.25000 * |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.470 |
| Number of reflections | 94192 | |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 98.7 | 92.3 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 17 * | GROWTH IN 20% PEG 3350, 0.2M TRIS-HCL PH 8.5. RESERVOIR EXCHANGED FOR 31% PEG 3350, 31% PEG 3350, 0.2M TRIS-HCL PH 8.5, FROZEN IN 31% PEG 3350, 0.2M TRIS-HCL PH 8.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | GTP | 10000 (nM) | |
| 6 | 1 | drop | protein | 11 (mg/ml) | |
| 7 | 1 | reservoir | PEG3350 | 20 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 0.2 (M) | pH8.5 |
