1UPF

STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE, MUTANT C128V BOUND TO THE DRUG 5-FLUOROURACIL

Experimental procedure

Temperature [K]	298
Collection date	1997-01
Spacegroup name	P 1 21 1
Unit cell lengths	60.460, 141.780, 71.460
Unit cell angles	90.00, 115.03, 90.00

Refinement procedure

Resolution	10.000 - 2.300
Rwork	0.166
RMSD bond length	0.015
RMSD bond angle	2.258
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.300
Rmerge	0.052 *
Total number of observations	91812 *
Number of reflections	62830 *
Completeness [%]	86.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	protein	25-40 (mg/ml)
4	1	reservoir	ammonium phosphate	1.0 (M)
5	1	reservoir	citrate	0.1 (M)
6	1	reservoir		0.2 (M)