1UM2
Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-17 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 0.7000 |
Spacegroup name | P 1 |
Unit cell lengths | 66.400, 70.400, 58.000 |
Unit cell angles | 100.40, 98.70, 78.90 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.217 |
Rwork | 0.214 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jva |
RMSD bond length | 0.012 |
RMSD bond angle | 1.720 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.051 | 0.141 |
Number of reflections | 20700 | |
Completeness [%] | 83.0 | 60.1 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 6000, BistrisHCl, 2-mercaptoethanol, magnesium chloride, cadmium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |