1UM2
Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.7000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.400, 70.400, 58.000 |
| Unit cell angles | 100.40, 98.70, 78.90 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jva |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.720 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.051 | 0.141 |
| Number of reflections | 20700 | |
| Completeness [%] | 83.0 | 60.1 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 6000, BistrisHCl, 2-mercaptoethanol, magnesium chloride, cadmium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
