1UM2

Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDONUCLEASE PI-SCEI	polymer	454	50963.8	2	UniProt (P17255) Pfam (PF05203) Pfam (PF05204)	Saccharomyces cerevisiae (baker's yeast)	VMA1-DERIVED ENDONUCLEASE X10SNS
2	C, D (C, D)	21-mer from Vacuolar ATP synthase catalytic subunit A	polymer	21	2231.4	2	UniProt (P17255)	Saccharomyces cerevisiae (baker's yeast)	21-mer from VMA1-DERIVED ENDONUCLEASE X10SNS
3	E, F (A, B)	water	water		18.0	153	Chemie (HOH)

Sequence modifications

A, B: 284 - 737 (UniProt: P17255)

PDB	External Database	Details
Ser 284	Cys 284	engineered mutation
Asn 362	His 362	engineered mutation

C, D: 274 - 283 (UniProt: P17255)

PDB	External Database	Details
Met 273	-	initiating methionine

C, D: 738 - 747 (UniProt: P17255)

PDB	External Database	Details
Ser 738	Cys 738	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	106390.4
All*	Total formula weight	106390.4

*Water molecules are not included.