1U7T
Crystal Structure of ABAD/HSD10 with a Bound Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.000, 80.800, 110.000 |
Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Wild-type human ABAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 69169 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | MME-PEG 2000, sodium citrate, isopropanol, pH 6.0, vapor diffusion, temperature 298K |