1U46
Crystal Structure of the Unphosphorylated Kinase Domain of the Tyrosine Kinase ACK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.176, 43.401, 84.452 |
Unit cell angles | 90.00, 111.82, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.20201 |
Rwork | 0.199 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.705 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 37411 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG 4000, magnesium chloride, sodium chloride, HEPES, TRIS, TCEP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |