1U32
Crystal structure of a Protein Phosphatase-1: Calcineurin Hybrid Bound to Okadaic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-07-22 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 98.759, 98.759, 62.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.010 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1JK7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.076 | 0.369 |
Number of reflections | 21304 | |
<I/σ(I)> | 21.1 | 5.02 |
Completeness [%] | 97.3 | 99 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | lithium sulfate, tris, PEG 400, mercaptoethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |