1U2Z
Crystal structure of histone K79 methyltransferase Dot1p from yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.070, 146.275, 75.412 |
Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.200 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.302 | |
Number of reflections | 79720 | |
<I/σ(I)> | 23.2 | 4.1 |
Completeness [%] | 98.7 | 94.1 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | PEG 8000, Glycerol, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |