1TZG
Crystal structure of HIV-1 neutralizing human Fab 4E10 in complex with a 13-residue peptide containing the 4E10 epitope on gp41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-02-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979126 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.300, 45.100, 198.500 |
Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
Resolution | 43.000 - 2.200 |
Rwork | 0.217 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hkl |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 61572 | |
<I/σ(I)> | 16.7 | 2.3 |
Completeness [%] | 93.0 | 61.4 |
Redundancy | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | PEG 8000, sodium acetate, hexamine cobalt trichloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |