1TXD
Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2002-08-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.606, 45.859, 74.730 |
| Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
| Resolution | 24.850 - 2.130 |
| R-factor | 0.23676 |
| Rwork | 0.235 |
| R-free | 0.27433 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 36990 | |
| <I/σ(I)> | 21 | 3.3 |
| Completeness [%] | 98.3 | 93.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG3350, sodium chloride, sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
