1TSQ

CRYSTAL STRUCTURE OF AP2V SUBSTRATE VARIANT OF NC-P1 DECAMER PEPTIDE IN COMPLEX WITH V82A/D25N HIV-1 PROTEASE MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	220
Detector technology	IMAGE PLATE
Collection date	2002-07-12
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.771, 57.258, 60.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.990 - 2.000
R-factor	0.195
Rwork	0.195
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mtr
RMSD bond length	0.017
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.990
High resolution limit [Å]	2.000
Rmerge	0.075
Number of reflections	12492
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	SODIUM PHOSPHATE, SODIUM CITRATE, AMMONIUM SULPHATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K