1TQL

POLIOVIRUS POLYMERASE G1A MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-21
Detector	NOIR-1
Wavelength(s)	0.98
Spacegroup name	P 65
Unit cell lengths	127.780, 127.780, 113.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.237
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.074	0.504
Number of reflections	46290
<I/σ(I)>	13.8	2.3
Completeness [%]	99.2	96.1
Redundancy	6.04	4.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	sodium acetate, cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K