1TC2
TERNARY SUBSTRATE COMPLEX OF THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE FROM TRYPANOSOMA CRUZI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-04 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.420, 101.760, 51.920 |
Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.810 |
R-factor | 0.191 |
Rwork | 0.183 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tc1 |
RMSD bond length | 0.010 |
RMSD bond angle | 24.480 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.600 | 1.850 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.051 * | 0.320 * |
Number of reflections | 33026 | |
<I/σ(I)> | 20.8 | 3.8 |
Completeness [%] | 88.8 | 80.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | PRPP | 5 (mM) | |
3 | 1 | drop | HPP | 5 (mM) | |
4 | 1 | drop | bis-tris | 20 (mM) | |
5 | 1 | drop | 6 (mM) | ||
6 | 1 | reservoir | sodium acetate | 0.1 (M) | |
7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
8 | 1 | reservoir | PEG4000 | 30 (%) |