1T69
Crystal Structure of human HDAC8 complexed with SAHA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 80.761, 80.761, 105.615 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.710 - 2.910 |
Rwork | 0.249 |
R-free | 0.31027 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t67 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.281 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.076 | 0.540 |
Number of reflections | 8164 | |
<I/σ(I)> | 17 | 4 |
Completeness [%] | 95.0 | 96.5 |
Redundancy | 5.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 277 | PEG 8000, HEPES, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |