1T3N
Structure of the catalytic core of DNA polymerase Iota in complex with DNA and dTTP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Wavelength(s) | 0.97969, 0.97938, 0.96859 |
Spacegroup name | P 65 |
Unit cell lengths | 98.831, 98.831, 202.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.265 |
R-free | 0.28580 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.560 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 49675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | ||||
1 |