1T2A
Crystal structure of human GDP-D-mannose 4,6-dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.979174 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.595, 122.064, 139.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.930 - 1.840 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n7h |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.047 | 0.336 |
| Number of reflections | 129347 | |
| <I/σ(I)> | 19.7 | 2.81 |
| Completeness [%] | 98.3 | 97.9 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 21% PEG 4000, 0.1 M Tris pH 8.5, 0.2 M sodium acetate, 0.075 M BOG, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
