1T08
Crystal structure of beta-catenin/ICAT helical domain/unphosphorylated APC R3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.472, 96.712, 86.395 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.720 - 2.100 |
R-factor | 0.215 |
Rwork | 0.204 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m1e |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.296 |
Number of reflections | 47853 | |
<I/σ(I)> | 15.2 | 2.16 |
Completeness [%] | 95.2 | 90.5 |
Redundancy | 2.67 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 297 | PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K |
1 | EVAPORATION | 6.5 | 297 | PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K |
1 | EVAPORATION | 6.5 | 297 | PEG3400, Potassium phosphate, pH 6.5, EVAPORATION, temperature 297K |