1SQ0
Crystal Structure of the Complex of the Wild-type Von Willebrand Factor A1 domain and Glycoprotein Ib alpha at 2.6 Angstrom Resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97942, 0.97959, 0.9686 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.497, 116.104, 67.962 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.580 - 2.600 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.580 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 17150 | |
Completeness [%] | 84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG-3350; potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |